Keratin 5/8 antibody

Catalog No. ABIN94292

The Mouse Monoclonal anti-Keratin 5/8 antibody has been validated for WB, IHC (p), ICC and IP. It is suitable to detect Keratin 5/8 in samples from Human, Mouse, Pig, Rat, Dog, Hamster, Cow and Sheep. There is 1 publication available.

Quick Overview for Keratin 5/8 antibody (ABIN94292)

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Pig, Rat, Dog, Hamster, Cow, Sheep

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C-50

Product Details anti-Keratin 5/8 Antibody

Purpose: Anti-Cytokeratin 5/8 Purified

Specificity: The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

No Cross-Reactivity: Chicken, Xenopus laevis

Cross-Reactivity (Details): Human, Sheep, Canine (Dog), Hamster, Mouse, Rat, Bovine, Porcine

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Cytoskeleton preparation from HeLa cells.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive control: human prostate.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Keratin 5/8 antibody (ABIN94292)

Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, (1991) (PubMed).

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: Cytokeratin 5/8

Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K5, K8, CK5, CK8, CYK5, CYK8, KRT5, KRT8