p53 antibody (Tumor Protein P53) (AA 378-393)

Details for Product anti-TP53 Antibody No. ABIN94450
Antigen
Epitope
AA 378-393, pSer392
118
75
75
64
63
62
51
51
50
48
40
39
39
37
31
31
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25
22
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2
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1
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Reactivity
Human
2161
483
466
251
91
65
62
33
25
21
19
14
13
7
6
5
4
4
4
4
3
3
3
3
2
2
1
Host
Mouse
1558
667
7
5
1
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This p53 antibody is un-conjugated
84
54
41
40
40
39
39
39
38
25
22
21
12
12
12
12
12
8
7
7
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7
7
7
3
3
3
3
3
3
3
3
3
3
2
2
2
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2
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2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1712
742
712
526
387
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282
155
99
84
61
49
41
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32
28
20
19
15
15
6
3
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2
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1
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Options
Immunogen KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
Clone FP3-2 [FPS392]
Isotype IgG1
Specificity The antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor protein phosphorylated at CKII site (Ser 392).
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Tumor Protein P53 (TP53) (AA 378-393), (pSer392) antibody (ABIN94450) Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraff...
Image no. 2 for anti-Tumor Protein P53 (TP53) (AA 378-393), (pSer392) antibody (ABIN94450) Immunohistochemistry staining of Wild-type p53 expressed in human trophoblast (paraff...
Product cited in: Blaydes, Craig, Wallace, Ball, Traynor, Gibbs, Hupp: "Synergistic activation of p53-dependent transcription by two cooperating damage recognition pathways." in: Oncogene, Vol. 19, Issue 34, pp. 3829-39, 2000 (PubMed).