Amylase beta antibody

Catalog No. ABIN95010

Product Details anti-Amylase beta Antibody

Target: Amylase beta (amyB)

Reactivity: Sweet potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against b Amylase from other sources may occur but has not been specifically determined.

Purity: Anti-BETA AMYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified b Amylase [Sweet Potato].

Endotoxin Level: Low Endotoxin : No

Immunogen: Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: BETA AMYLASE antibody has been assayed against 1.0 µg of b Amylase [Sweet Potato] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:16.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Amylase beta

Target: Amylase beta (amyB)

Alternative Name: BETA AMYLASE (amyB Products)

Synonyms: beta-amylase antibody, Beta-amylase antibody, CBO1203 antibody, amyB antibody, CLM_1356 antibody, BMQ_0356 antibody, BMD_0357 antibody

Background: Synonyms: 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

UniProt: P10537