Choline Acetyltransferase antibody

Catalog No. ABIN951492

This anti-Choline Acetyltransferase antibody is a Chicken Polyclonal antibody detecting Choline Acetyltransferase in IF and IHC (p). Suitable for Human and Mouse.

Quick Overview for Choline Acetyltransferase antibody (ABIN951492)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Choline Acetyltransferase Antibody

Specificity: Recognizes Choline Acetyltransferase (ChAT).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Synthetic peptide KLH conjugated corresponding to a region of the Choline Acetyltransferase gene product shared between the Human (P28329) and Mouse (Q03059) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: CHAT

Background: Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).Synonyms: CHOACTase, ChAT, Choline O-acetyltransferase, Choline acetylase, EC=2.3.1.6

Gene ID: 1103

NCBI Accession: NP_001136401

Pathways: Skeletal Muscle Fiber Development