FBXW7 antibody (AA 599-708)

Catalog No. ABIN952295

The Mouse Monoclonal anti-FBXW7 antibody is suitable to detect FBXW7 in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for FBXW7 antibody (AA 599-708) (ABIN952295)

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FBXW7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 3D1

Product Details anti-FBXW7 Antibody

Binding Specificity: AA 599-708

Specificity: Recognizes Fbxw7

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography

Immunogen: FBXW7 (NP_361014, 599 a.a. ~ 708 a.a) partial recombinant protein with GST tag.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FBXW7

Target: FBXW7 (F-Box and WD Repeat Domain Containing 7 (FBXW7))

Alternative Name: FBXW7

Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.Synonyms: Archipelago homolog, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW7, FBX30, SEL-10, SEL10, hAgo, hCDC4

Gene ID: 55294

NCBI Accession: NP_001013433

UniProt: Q969H0

Pathways: Notch Signaling, EGFR Signaling Pathway