HLA B7 antibody (Middle Region)

Catalog No. ABIN952748

Product Details anti-HLA B7 Antibody

Target: HLA B7 (HLA Class I B7 Alpha (HLA B7))

Binding Specificity: AA 184-212, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA B7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human HLA class I B7 alpha / HLA-B (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human HLA-B

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HLA B7

Target: HLA B7 (HLA Class I B7 Alpha (HLA B7))

Abstract: HLA B7 Products

Synonyms: AS antibody, HLAB antibody, SPDA1 antibody, major histocompatibility complex, class I, B antibody, HLA-B antibody

Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.Synonyms: B-7 alpha chain, HLA class I histocompatibility antigen, HLAB, MHC class I antigen B7

Molecular Weight: 40460 Da

Gene ID: 3106

NCBI Accession: NP_005505