HLA-DRB5 antibody (Middle Region)

Catalog No. ABIN952755

Product Details anti-HLA-DRB5 Antibody

Target: HLA-DRB5 (Major Histocompatibility Complex, Class II, DR beta 5 (HLA-DRB5))

Binding Specificity: AA 49-79, Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DRB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human HLA class II DRB5 beta / HLA-DRB5 (Center).

Purification: Protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide between 49~79 amino acids from the Central region of human HLA class II DRB5 beta / HLA-DRB5

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HLA-DRB5

Target: HLA-DRB5 (Major Histocompatibility Complex, Class II, DR beta 5 (HLA-DRB5))

Alternative Name: HLA Class II DRB5 beta / HLA-DRB5 (HLA-DRB5 Products)

Synonyms: HLA-DRB antibody, major histocompatibility complex, class II, DR beta 5 antibody, HLA-DRB5 antibody

Background: HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.Synonyms: DR beta-5, DR2-beta-2, DRB5 beta chain, Dw2, HLA class II histocompatibility antigen, MHC class II antigen DRB5

Molecular Weight: 30056 Da

Gene ID: 3127

NCBI Accession: NP_002116

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints