MAPT antibody

Catalog No. ABIN953316

The Chicken Polyclonal anti-MAPT antibody (ABIN953316) specifically detects MAPT in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for MAPT antibody (ABIN953316)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human, Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPT Antibody

Specificity: Recognizes Tau.

Purification: Affinity Chromatography.

Immunogen: Antipeptide antibodies were generated in chickens against two sequences shared between the Mouse (P10637.3) and Human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL (0.1 mg/mL of each antibody)

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT / tau

Background: Human Tau protein (a.k.a., Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

Gene ID: 4137

NCBI Accession: NP_001116538

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size