ELANE antibody (N-Term)

Catalog No. ABIN953688

The Rabbit Polyclonal anti-ELANE antibody (ABIN953688) specifically detects ELANE in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for ELANE antibody (N-Term) (ABIN953688)

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELANE antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ELANE Antibody

Binding Specificity: AA 20-47, N-Term

Specificity: This antibody recognizes human Neutrophil elastase at N-term.

Purification: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 20-47aa ) of human Neutrophil elastase

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Alternative Name: Neutrophil Elastase

Background: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.Synonyms: Bone marrow serine protease, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase

Molecular Weight: 28518 Da

Gene ID: 1991

NCBI Accession: NP_001963