RAD17 antibody (Middle Region)

Catalog No. ABIN954430

This Rabbit Polyclonal antibody specifically detects RAD17 in WB, FACS and EIA. It exhibits reactivity toward Human.

Quick Overview for RAD17 antibody (Middle Region) (ABIN954430)

Target: RAD17

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD17 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Product Details anti-RAD17 Antibody

Binding Specificity: AA 225-254, Middle Region

Specificity: This antibody recognizes Human RAD17 (Center).

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of Human RAD17

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAD17

Target: RAD17

Alternative Name: RAD17

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.Synonyms: Cell cycle checkpoint protein RAD17, R24L, RF-C/activator 1 homolog

Molecular Weight: 77055 Da

Gene ID: 5884

NCBI Accession: NP_002864