USP28 antibody (Internal Region)

Catalog No. ABIN955488

Product Details anti-USP28 Antibody

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Binding Specificity: Internal Region

Reactivity: Human, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This USP28 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-SVELKHYIQE DN

Specificity: Recognizes USP28

Cross-Reactivity (Details): Species reactivity (expected):Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_065937.1. Genename: USP28

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for USP28

Target: USP28 (Ubiquitin Specific Peptidase 28 (USP28))

Alternative Name: USP28 (USP28 Products)

Synonyms: im:7138195 antibody, USP28 antibody, 9830148O20Rik antibody, AU022237 antibody, mKIAA1515 antibody, ubiquitin specific peptidase 28 antibody, USP28 antibody, usp28 antibody, Usp28 antibody

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 28, KIAA1515, Ubiquitin carboxyl-terminal hydrolase 28, Ubiquitin thioesterase 28, Ubiquitin-specific-processing protease 28

Gene ID: 57646

NCBI Accession: NP_065937