BDNF antibody

Catalog No. ABIN964574

Supplier Product No.: 209-401-c27

Product Details anti-BDNF Antibody

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 209-401-c27

Supplier: Rockland

Purpose: BDNF Antibody

Cross-Reactivity (Details): This antibody is specific for human BDNF protein.

Characteristics: Synonyms: rabbit anti-BDNF, rabbit anti-brain derived neurotropic factor, Brain-derived neurotrophic factor, Abrineurin cytokine

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated human BDNF protein produced in E.coli.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 27 kDa in size corresponding to the mature human BDNF protein by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF (BDNF Products)

Background: Background: BDNF is a member of the nerve growth factor family of trophic factors. In the brain BDNF has a trophic action on retinal, cholinergic, and dopaminergic neurons, and in the peripheral nervous system it acts on both motor and sensory neurons. Some protein domains of BDNF are identical with those of NGF and another neurotrophic factor, designated NT-3 (neurotrophin-3). It exists as monomers and homodimers, and binds to NTRK2/TRKB. Polyclonal antibodies raised against murine NGF have been shown to cross-react with both NT-3 and BDNF. The propeptide is N-glycosylated and glycosulfated. BDNF is converted into mature BDNF by plasmin (PLG). It is expressed in brain, and highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum, it is also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Gene ID: 627

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity