Surface Lipoprotein p27 antibody
Reactivity: Borrelia burgdorferi
LF, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
-
- Target
- Surface Lipoprotein p27
- Reactivity
- Borrelia burgdorferi
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Lateral Flow (LF), Western Blotting (WB)
- Specificity
- This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography, and cross-adsorbed against MBP. It is directed against, and shows specific reactivity for, Borrelia burgdorferi p27 protein. Reactivity with p27 protein from other sources has not been determined.
- Characteristics
- Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals.
- Purification
- purified
- Sterility
- Sterile filtered
- Immunogen
-
MBP-fusion protein corresponding to Borrelia burgdorferi Surface Lipoprotein p27 protein.
Immunogen Type: RecombinantProtein - Isotype
- IgG
-
- Application Notes
- Anti-Surface Lipoprotein p27 antibody has been tested in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~30.9 kDa in size corresponding to p27 by Western blotting in the appropriate cell lysate or extract.
- Comment
-
Gene Name: BB_A60
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Expiry Date
- 12 months
-
- Target
- Surface Lipoprotein p27
- Background
-
Surface Lipoprotein p27 of Borrelia burgdorferi is a surface-exposed lipoprotein that has been shown (by Western blot and Northern blot) to be expressed in the European B. burgdorferi strain B29, but not in the American strain B31. Cell envelope proteins of bacterial pathogens play important roles in the host-parasite interactions that occur during infection, including cell adherence, cell invasion, and immune cell activation or evasion. p27 is a basic protein of 248 amino acids with a typical prokaryotic leader sequence of 17 amino acid residues at the N-terminus of the proposed translation product. The p27 gene is located on a linear plasmid of a size of approximately 55 kb. Borrelia spirochetes are unique among diderm bacteria in their abundance of surface-displayed lipoproteins, some of which play important roles in the pathogenesis of Lyme disease and relapsing fever. There is evidence that Borrelia lipoproteins are specifically targeted to the bacterial surface, but that they can be retained in the periplasm by sequence-specific signals.
Synonyms: BBA060 protein, Borrelia burgdorferi p27, Surface lipoprotein p27 - Gene ID
- 1194336
- NCBI Accession
- NP_0457331
- UniProt
- O50951
-
You are here:
