Goat anti-Rat IgG (Heavy & Light Chain) Antibody (Atto 647N) - Preadsorbed

Catalog No. ABIN965007

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: Atto 647N

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.

Characteristics: Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Rat IgG whole molecule

Isotype: IgG

Labeling Ratio: 1.68

Application Details

Application Notes:

Application Note: The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

Western Blot Dilution: >1:10,000

IF Microscopy Dilution: >1:5,000

Comment:

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

References

Meissner, Grimm, Johnston, Sutcliffe, Ng, Jefferis, Cachero, Lavis, Malkesman: "Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons." in: PLoS ONE, Vol. 13, Issue 8, pp. e0200759, (2019) (PubMed).

Dolan, Frechter, Bates, Dan, Huoviala, Roberts, Schlegel, Dhawan, Tabano, Dionne, Christoforou, Close, Sutcliffe, Giuliani, Li, Costa, Ihrke, Meissner, Bock, Aso, Rubin, Jefferis: "Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body." in: eLife, Vol. 8, (2019) (PubMed).

Turner-Evans, Wegener, Rouault, Franconville, Wolff, Seelig, Druckmann, Jayaraman: "Angular velocity integration in a fly heading circuit." in: eLife, Vol. 6, (2018) (PubMed).

Wolff, Rubin: "Neuroarchitecture of the Drosophila central complex: A catalog of nodulus and asymmetrical body neurons and a revision of the protocerebral bridge catalog." in: The Journal of comparative neurology, Vol. 526, Issue 16, pp. 2585-2611, (2018) (PubMed).

von Reyn, Nern, Williamson, Breads, Wu, Namiki, Card: "Feature Integration Drives Probabilistic Behavior in the Drosophila Escape Response." in: Neuron, Vol. 94, Issue 6, pp. 1190-1204.e6, (2017) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat anti-Rat IgG ATTO647N Conjugated Antibody, Goat anti-Rat IgG Antibody ATTO 647N Conjugation

Background: Anti-Rat IgG (H&L) conjugated to ATTO 647N is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.