Smooth Muscle Actin antibody

Catalog No. ABIN965507

The Mouse Monoclonal anti-Smooth Muscle Actin antibody has been validated for WB, IHC and ELISA. It is suitable to detect Smooth Muscle Actin in samples from Human, Rat, Mouse and Monkey. There is 1 publication available.

Quick Overview for Smooth Muscle Actin antibody (ABIN965507)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 4A4

Product Details anti-Smooth Muscle Actin Antibody

Purification: Ascitic fluid

Immunogen: Synthesized peptide of human ACTA2.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Smooth Muscle Actin antibody (ABIN965507)

Morisaki, Akutsu, Ogino, Kondo, Yamanaka, Tsutsumi, Yoshimuta, Okajima, Matsuda, Minatoya, Sasaki, Tanaka, Ishibashi-Ueda, Morisaki: "Mutation of ACTA2 gene as an important cause of familial and nonfamilial nonsyndromatic thoracic aortic aneurysm and/or dissection (TAAD)." in: Human mutation, Vol. 30, Issue 10, pp. 1406-11, (2009) (PubMed).

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Alternative Name: ACTA2

Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Synonyms: AA76, ACTSA, ASmooth Muscle Actin.

Molecular Weight: 42KDa

Gene ID: 59

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development