Aurora A antibody

Catalog No. ABIN965625

This anti-Aurora A antibody is a Mouse Monoclonal antibody detecting Aurora A in WB, ELISA, IF and FACS. Suitable for Human, Rat and Monkey. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Aurora A antibody (ABIN965625)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 1F8

Product Details anti-Aurora A Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human AURKA expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Aurora A antibody (ABIN965625)

De Luca, Brunetto, Asteriti, Giubettini, Lavia, Guarguaglini: "Aurora-A and ch-TOG act in a common pathway in control of spindle pole integrity." in: Oncogene, Vol. 27, Issue 51, pp. 6539-49, (2008) (PubMed).

Veerakumarasivam, Goldstein, Saeb-Parsy, Scott, Warren, Thorne, Mills, Venkitaraman, Neal, Kelly: "AURKA overexpression accompanies dysregulation of DNA-damage response genes in invasive urothelial cell carcinoma." in: Cell cycle (Georgetown, Tex.), Vol. 7, Issue 22, pp. 3525-33, (2008) (PubMed).

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA

Background: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.
Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Synonyms: AIK, ARK1, AURA, BTAK, STK6,

Molecular Weight: 48kDa

Gene ID: 6790

Pathways: Cell Division Cycle, Asymmetric Protein Localization