BRCA1 antibody

Catalog No. ABIN965696

There are 5+ publications for this product available. The Rabbit Polyclonal anti-BRCA1 antibody is suitable to detect BRCA1 in samples from Human. It has been validated for IHC.

Quick Overview for BRCA1 antibody (ABIN965696)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-BRCA1 Antibody

Specificity: BRCA1 (Ab-1423) antibody detects endogenous levels of total BRCA1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1423 (H-G-SP-Q-P).

Application Details

Application Notes: IHC: 1:50-1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-BRCA1 antibody (ABIN965696)

Greenberg, Sobhian, Pathania, Cantor, Nakatani, Livingston: "Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes." in: Genes & development, Vol. 20, Issue 1, pp. 34-46, (2006) (PubMed).

Arlt, Xu, Durkin, Casper, Kastan, Glover: "BRCA1 is required for common-fragile-site stability via its G2/M checkpoint function." in: Molecular and cellular biology, Vol. 24, Issue 15, pp. 6701-9, (2004) (PubMed).

Zhang, Willers, Feng, Ghosh, Kim, Weaver, Chung, Powell, Xia: "Chk2 phosphorylation of BRCA1 regulates DNA double-strand break repair." in: Molecular and cellular biology, Vol. 24, Issue 2, pp. 708-18, (2003) (PubMed).

Kim, Xu, Kastan: "Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage." in: Genes & development, Vol. 16, Issue 5, pp. 560-70, (2002) (PubMed).

Tibbetts, Cortez, Brumbaugh, Scully, Livingston, Elledge, Abraham: "Functional interactions between BRCA1 and the checkpoint kinase ATR during genotoxic stress." in: Genes & development, Vol. 14, Issue 23, pp. 2989-3002, (2000) (PubMed).

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus