CDK2 antibody

Catalog No. ABIN965843

There are 4+ publications for this product available. The Rabbit Polyclonal anti-CDK2 antibody is suitable to detect CDK2 in samples from Human, Mouse and Rat. It has been validated for IHC.

Quick Overview for CDK2 antibody (ABIN965843)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-CDK2 Antibody

Specificity: CDK2 (Ab-160) antibody detects endogenous levels of total CDK2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human CDK2 around the phosphorylation site of threonine160 (T-Y-TP-H-E).

Application Details

Application Notes: WB: 1:500~1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-CDK2 antibody (ABIN965843)

Liu, Wu, Galaktionov: "p42, a novel cyclin-dependent kinase-activating kinase in mammalian cells." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4507-14, (2004) (PubMed).

Ukomadu, Dutta: "Inhibition of cdk2 activating phosphorylation by mevastatin." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4840-6, (2003) (PubMed).

Morris, Gondeau, Tainer, Divita: "Kinetic mechanism of activation of the Cdk2/cyclin A complex. Key role of the C-lobe of the Cdk." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23847-53, (2002) (PubMed).

Brown, Noble, Lawrie, Morris, Tunnah, Divita, Johnson, Endicott: "Effects of phosphorylation of threonine 160 on cyclin-dependent kinase 2 structure and activity." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8746-56, (1999) (PubMed).

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA