Estrogen Receptor alpha antibody (AA 410-592)

Catalog No. ABIN966081

Product Details anti-Estrogen Receptor alpha Antibody

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Binding Specificity: AA 410-592

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purification: Purified

Immunogen: Purified recombinant fragment of human ER-alpha (410-592aa) expressed in E. Coli.

Clone: 2B7

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: PBS

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Estrogen Receptor alpha antibody (ABIN966081)

Pertschuk, Feldman, Kim, Braithwaite, Schneider, Braverman, Axiotis: "Estrogen receptor immunocytochemistry in paraffin embedded tissues with ER1D5 predicts breast cancer endocrine response more accurately than H222Sp gamma in frozen sections or cytosol-based ligand-binding assays." in: Cancer, Vol. 77, Issue 12, pp. 2514-9, (1996) (PubMed).

Pertschuk, Masood, Simone, Feldman, Fruchter, Axiotis, Greene: "Estrogen receptor immunocytochemistry in endometrial carcinoma: a prognostic marker for survival." in: Gynecologic oncology, Vol. 63, Issue 1, pp. 28-33, (1996) (PubMed).

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ER-alpha (ESR1 Products)

Background: The estrogen receptor (ER) is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Alternative splicing results in several ER mRNA transcripts, which differ primarily in their 5-prime untranslated regions. Two isoforms of the human ER, ERA and ER-beta, occur, each with distinct tissue and cell patterns of expression. Pelletier and El-Alfy (2000) studied the immunocytochemical localization of ESRA and ESRB in human reproductive tissues. In the ovary, ERB immunoreactivity was found in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, interstitial gland, and germinal epithelium cells. Nuclear staining for ERA occurred in thecal, interstitial gland, and germinal epithelium cells. In the uterus, strong ERA immunoreactivity was detected in nuclei of epithelial, stromal, and muscle cells.
Synonyms: ER, ESR1, Era, ESRA

Gene ID: 2099

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly