EpCAM antibody

Catalog No. ABIN966093

Cited in 1 publication. The Mouse Monoclonal anti-EpCAM antibody (Clone B29-1 (VU-ID9)) (ABIN966093) specifically detects EpCAM in IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for EpCAM antibody (ABIN966093)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B29-1 (VU-ID9)

Product Details anti-EpCAM Antibody

Purification: Concentrated.

Immunogen: Small Cell Lung Carcinoma Cells

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:60.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:60 with AutoProbe III. The optimal conditions should be determined by the individual laboratory.To differentiate between mesothelioma and carcinoma.

Comment:

Cellular Localization: Cell Membrane.
Recommended Positive Control: Breast, Colon Carcinomas, and Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-EpCAM antibody (ABIN966093)

Szala, Froehlich, Scollon, Kasai, Steplewski, Koprowski, Linnenbach: "Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 9, pp. 3542-6, (1990) (PubMed).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: ESA

Background: This antibody reacts with a 40 kD cell surface glycoprotein called epithelial specific antigen (ESA). It reacts with the same molecule which is also detected by antibodies such as 17-1A, AUA1, KS1/4, Ber-EP4, 323/A3, MOC-31, GA733, HEA125 and others.

Gene ID: 4072