GSK3 alpha antibody

Catalog No. ABIN966231

This anti-GSK3 alpha antibody is a Monoclonal antibody detecting GSK3 alpha in WB and ELISA. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for GSK3 alpha antibody (ABIN966231)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GSK3 alpha Antibody

Specificity: Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3)

Purification: Crude ascites.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-GSK3 alpha antibody (ABIN966231)

Mendez, Garcia-Segura: "Phosphatidylinositol 3-kinase and glycogen synthase kinase 3 regulate estrogen receptor-mediated transcription in neuronal cells." in: Endocrinology, Vol. 147, Issue 6, pp. 3027-39, (2006) (PubMed).

Bianchi, De Lucchini, Marin, Turner, Hanks, Villa-Moruzzi: "Regulation of FAK Ser-722 phosphorylation and kinase activity by GSK3 and PP1 during cell spreading and migration." in: The Biochemical journal, Vol. 391, Issue Pt 2, pp. 359-70, (2005) (PubMed).

De Servi, Hermani, Medunjanin, Mayer: "Impact of PKCdelta on estrogen receptor localization and activity in breast cancer cells." in: Oncogene, Vol. 24, Issue 31, pp. 4946-55, (2005) (PubMed).

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha

Background: : Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Gene ID: 11076

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect