MEK1 (Ab-291) antibody detects endogenous levels of total MEK1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291 (P-R-TP-P-G).
MAP2K1
Reactivity: Human
WB, IHC, ELISA, IP
Host: Rabbit
Monoclonal
3F10
unconjugated
Recombinant Antibody
Application Notes
WB: 1:500~1:1000 IHC: 1:50~1:100.
Restrictions
For Research Use only
Format
Liquid
Concentration
100ug/100ul.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Burroughs, Oh, Barrett, DiAugustine et al.: "Phosphatidylinositol 3-kinase and mek1/2 are necessary for insulin-like growth factor-I-induced vascular endothelial growth factor synthesis in prostate epithelial cells: a role for hypoxia-inducible ..." in: Molecular cancer research : MCR, Vol. 1, Issue 4, pp. 312-22, (2003) (PubMed).
Schramek, Feifel, Marschitz, Golochtchapova, Gstraunthaler, Montesano: "Loss of active MEK1-ERK1/2 restores epithelial phenotype and morphogenesis in transdifferentiated MDCK cells." in: American journal of physiology. Cell physiology, Vol. 285, Issue 3, pp. C652-61, (2003) (PubMed).
Piatelli, Doughty, Chiles: "Requirement for a hsp90 chaperone-dependent MEK1/2-ERK pathway for B cell antigen receptor-induced cyclin D2 expression in mature B lymphocytes." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 12144-50, (2002) (PubMed).
Morgan, Labno, Van Seventer, Denny, Straus, Burkhardt: "Superantigen-induced T cell:B cell conjugation is mediated by LFA-1 and requires signaling through Lck, but not ZAP-70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 10, pp. 5708-18, (2001) (PubMed).
Target
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))