MEK1 antibody

Catalog No. ABIN966565

This Rabbit Polyclonal antibody specifically detects MEK1 in IHC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for MEK1 antibody (ABIN966565)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MEK1 Antibody

Specificity: MEK2 (Ab-394) antibody detects endogenous levels of total MEK2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human MEK2 around the phosphorylation site of threonine 394 (P-G-TP-P-T).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-MEK1 antibody (ABIN966565)

Alessi, Saito, Campbell, Cohen, Sithanandam, Rapp, Ashworth, Marshall, Cowley: "Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1." in: The EMBO journal, Vol. 13, Issue 7, pp. 1610-9, (1994) (PubMed).

Rosen, Ginty, Weber, Greenberg: "Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras." in: Neuron, Vol. 12, Issue 6, pp. 1207-21, (1994) (PubMed).

Cowley, Paterson, Kemp, Marshall: "Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells." in: Cell, Vol. 77, Issue 6, pp. 841-52, (1994) (PubMed).

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling