PRKDC antibody

Catalog No. ABIN966893

Product Details anti-PRKDC Antibody

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to middle residues of human PRKDC(DNA-dependent protein kinase catalytic subunit)

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-PRKDC antibody (ABIN966893)

Ladenburger, Fackelmayer, Hameister, Knippers: "MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13." in: Cytogenetics and cell genetics, Vol. 77, Issue 3-4, pp. 268-70, (1997) (PubMed).

Sipley, Menninger, Hartley, Ward, Jackson, Anderson: "Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7515-9, (1995) (PubMed).

Poltoratsky, Shi, York, Lieber, Carter: "Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4529-33, (1995) (PubMed).

Liu, Ma, Yueh, Lees-Miller, Anderson, Ng: "The carboxyl-terminal transactivation domain of human serum response factor contains DNA-activated protein kinase phosphorylation sites." in: The Journal of biological chemistry, Vol. 268, Issue 28, pp. 21147-54, (1993) (PubMed).

Iijima, Teraoka, Date, Tsukada: "DNA-activated protein kinase in Raji Burkitt's lymphoma cells. Phosphorylation of c-Myc oncoprotein." in: European journal of biochemistry / FEBS, Vol. 206, Issue 2, pp. 595-603, (1992) (PubMed).

Carter, Vancurová, Sun, Lou, DeLeon: "A DNA-activated protein kinase from HeLa cell nuclei." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6460-71, (1991) (PubMed).

Lees-Miller, Anderson: "The human double-stranded DNA-activated protein kinase phosphorylates the 90-kDa heat-shock protein, hsp90 alpha at two NH2-terminal threonine residues." in: The Journal of biological chemistry, Vol. 264, Issue 29, pp. 17275-80, (1989) (PubMed).

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: PRKDC (PRKDC Products)

Synonyms: DNA-PKcs antibody, DNAPK antibody, DNPK1 antibody, HYRC antibody, HYRC1 antibody, XRCC7 antibody, p350 antibody, DNA-PKCS antibody, dna-pkcs antibody, dnapk antibody, dnpk1 antibody, hyrc antibody, hyrc1 antibody, prkdc-A antibody, xrcc7 antibody, PRKDC antibody, AI326420 antibody, AU019811 antibody, DNAPDcs antibody, DOXNPH antibody, dxnph antibody, p460 antibody, scid antibody, slip antibody, protein kinase, DNA-activated, catalytic polypeptide antibody, protein kinase, DNA-activated, catalytic polypeptide L homeolog antibody, DNA-dependent protein kinase catalytic subunit antibody, protein kinase, DNA activated, catalytic polypeptide antibody, PRKDC antibody, prkdc.L antibody, LOC100633457 antibody, Prkdc antibody

Background: PRKDC(DNA-dependent protein kinase catalytic subunit) is a Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. PRKDC must be bound to DNA to express its catalytic properties. PRKDC promotes processing of hairpin DNA structures in V(D)Jrecombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. PRKDC is required to protect and align broken ends of DNA. PRKDC may also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. PRKDC found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. PRKDC also involved in modulation of transcription. PRKDC recognizes the substrate consensus sequence [ST]-Q. PRKDC phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. PRKDC phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2. PRKDC can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate TP53/p53 in the presence of supercoiled DNA is dependent on C1D.

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response