TNF alpha antibody (AA 115-130)

Catalog No. ABIN967215

The Mouse Monoclonal anti-TNF alpha antibody (Clone B428) (ABIN967215) specifically detects TNF alpha in FACS and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (AA 115-130) (ABIN967215)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B428

Product Details anti-TNF alpha Antibody

Binding Specificity: AA 115-130

Specificity: This antibody reacts with TNFa.

Purification: Concentrated.

Immunogen: Synthetic TNF-alpha 115-130 hexadecapeptide linked to carrier protein.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
ELISA 1:500 -1:1000 (this antibody is not purified and can not be used to coat ELISA plates) .
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic and extracellular.
Recommended Positive Control: Prostate cancer

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor Alpha

Molecular Weight: 17 kDa

Gene ID: 7124

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome