p53 antibody

Catalog No. ABIN967457

The Mouse Monoclonal anti-p53 antibody has been validated for WB, IP, IHC (f) and IHC (fro). It is suitable to detect p53 in samples from Human and Cow. There are 5+ publications available.

Quick Overview for p53 antibody (ABIN967457)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: DO

Product Details anti-p53 Antibody

Brand: BD Pharmingen™

Cross-Reactivity: Cow (Bovine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human p53

Isotype: IgG2a

Application Details

Application Notes: Applications include immunoprecipitation (1-2 µg/1x10^6 cells), western blot analysis (2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651), SK-BR-3 human breast carcinoma cells (ATCC HTB-30), and A431 human vulval carcinoma cells (ATCC CRL-1555). Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach or breast tissue.

Comment:

Related Products: ABIN967389, ABIN968533

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-p53 antibody (ABIN967457)

Friedrichs, Gluba, Eidtmann, Jonat: "Overexpression of p53 and prognosis in breast cancer." in: Cancer, Vol. 72, Issue 12, pp. 3641-7, (1994) (PubMed).

Xerri, Bouabdallah, Camerlo, Hassoun: "Expression of the p53 gene in Hodgkin's disease: dissociation between immunohistochemistry and clinicopathological data." in: Human pathology, Vol. 25, Issue 5, pp. 449-54, (1994) (PubMed).

Bartkova, Bartek, Vojtesek, Lukas, Rejthar, Kovarik, Millis, Lane, Barnes: "Immunochemical analysis of the p53 oncoprotein in matched primary and metastatic human tumours." in: European journal of cancer (Oxford, England : 1990), Vol. 29A, Issue 6, pp. 881-6, (1993) (PubMed).

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, (1992) (PubMed).

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, (1991) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background: The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wild-type p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wild type p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. DO-1 reacts with human wild-type and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-1 recognizes an epitope between amino acids 1 and 45 of all known forms of human p53. Human recombinant p53 protein was used as immunogen.

Molecular Weight: 53 kDa

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect