Annexin a1 antibody (AA 1-346)

Catalog No. ABIN967719

Cited in 4+ publications. The Mouse Monoclonal anti-Annexin a1 antibody (Clone 29-Annexin I) (ABIN967719) specifically detects Annexin a1 in WB, IHC, IP and IF. The antibody is reactive with Human and Dog samples.

Quick Overview for Annexin a1 antibody (AA 1-346) (ABIN967719)

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Reactivity: Human, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin a1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 29-Annexin I

Product Details anti-Annexin a1 Antibody

Binding Specificity: AA 1-346

Cross-Reactivity: Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow Annexin I aa. 1-346

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Annexin a1 antibody (ABIN967719)

Hansen, Ehrlich, Nelson: "Molecular mechanism for orienting membrane and actin dynamics to nascent cell-cell contacts in epithelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45371-6, (2002) (PubMed).

Graham, Gerke, Burgoyne: "Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis." in: Molecular biology of the cell, Vol. 8, Issue 3, pp. 431-42, (1997) (PubMed).

Chasserot-Golaz, Vitale, Sagot, Delouche, Dirrig, Pradel, Henry, Aunis, Bader: "Annexin II in exocytosis: catecholamine secretion requires the translocation of p36 to the subplasmalemmal region in chromaffin cells." in: The Journal of cell biology, Vol. 133, Issue 6, pp. 1217-36, (1996) (PubMed).

Regnouf, Sagot, Delouche, Devilliers, Cartaud, Henry, Pradel: ""In vitro" phosphorylation of annexin 2 heterotetramer by protein kinase C. Comparative properties of the unphosphorylated and phosphorylated annexin 2 on the aggregation and fusion of chromaffin granule membranes." in: The Journal of biological chemistry, Vol. 270, Issue 45, pp. 27143-50, (1995) (PubMed).

Target Details for Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Alternative Name: Annexin I

Background: Annexin I, also known as lipocortin-1 or calpactin II, is part of a calcium-dependent phospholipid-binding protein family. Members share a common core domain, but each has a unique N-terminal tail that imparts its functional specificity. It is believed that phosphorlyation of this region in annexin I regulates its functions. Annexin I has been implicated in regulating phospholipid vesicle aggregation, mediating inflammatory response, and inhibiting the activity of phospholipase A2. Additionally, it has been found that the 10 kDa protein S100C influences annexin I's activity by binding to the N-terminal 13 residues of annexin in a manner similar to that of the annexin II/p11 complex.
Synonyms: Lipocortin-1, Calpactin II

Molecular Weight: 38 kDa

Pathways: Hormone Transport