RBP4 antibody (AA 1-135)

Catalog No. ABIN967847

Product Details anti-RBP4 Antibody

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Binding Specificity: AA 1-135

Reactivity: Human, Mouse, Rat, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Chicken, Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human RBP aa. 1-135

Clone: 42-RBP

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968619

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-RBP4 antibody (ABIN967847)

Christensen, Moskaug, Vorum, Jacobsen, Gundersen, Nykjaer, Blomhoff, Willnow, Moestrup: "Evidence for an essential role of megalin in transepithelial transport of retinol." in: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 4, pp. 685-95, (1999) (PubMed).

Zanotti, Berni, Monaco: "Crystal structure of liganded and unliganded forms of bovine plasma retinol-binding protein." in: The Journal of biological chemistry, Vol. 268, Issue 15, pp. 10728-38, (1993) (PubMed).

Zanotti, Malpeli, Berni: "The interaction of N-ethyl retinamide with plasma retinol-binding protein (RBP) and the crystal structure of the retinoid-RBP complex at 1.9-A resolution." in: The Journal of biological chemistry, Vol. 268, Issue 33, pp. 24873-9, (1993) (PubMed).

Zanotti, Ottonello, Berni, Monaco: "Crystal structure of the trigonal form of human plasma retinol-binding protein at 2.5 A resolution." in: Journal of molecular biology, Vol. 230, Issue 2, pp. 613-24, (1993) (PubMed).

Blomhoff, Green, Berg, Norum: "Transport and storage of vitamin A." in: Science (New York, N.Y.), Vol. 250, Issue 4979, pp. 399-404, (1990) (PubMed).

Target Details for RBP4

Target: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Alternative Name: RBP (RBP4 Products)

Synonyms: RDCCAS antibody, RBP antibody, RNBP antibody, rbp4 antibody, MGC54038 antibody, srbp antibody, PRBP antibody, RBPA antibody, Rbp-4 antibody, fb23c12 antibody, rbp antibody, wu:fb23c12 antibody, wu:fb58d04 antibody, wu:fb72b04 antibody, zgc:86911 antibody, retinol binding protein 4 antibody, renin binding protein antibody, retinol binding protein 4 S homeolog antibody, retinol binding protein 4 A, plasma antibody, retinol binding protein 4, plasma antibody, retinol binding protein 4 L homeolog antibody, RBP4 antibody, RENBP antibody, rbp4.S antibody, rbp4 antibody, Rbp4 antibody, RBP4A antibody, rbp4.L antibody

Background: Retinol, a metabolic precursor of retinal and retinoic acid, is transported in plasma bound to a carrier protein known as retinol-binding protein (RBP). RBP is the only retinoid-specific binding protein that has been identified in plasma. The presence of RBP increases the stability of retinol as it is transported from its stores to target cells. In plasma, the 21 kDa RBP forms a tight complex with thyroxine-binding transthyretin (TTR), a homotetramer of about 55 kDa. Association of RBP with TTR increases the stability of the retinol-RBP complex. It is believed that this association prevents filtration of the relatively small RBP through the kidney glomeruli. Once retinol is delivered to its target cells, the resulting retinol-free RBP has a reduced affinity for TTR and thus can be selectively cleared from the circulation by filtration through kidney glomeruli. The three dimensional structures of human and bovine RBP have been compared and appear to be very similar.

Molecular Weight: 21 kDa

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response