CNKSR1 antibody (AA 10-217)

Catalog No. ABIN968649

Product Details anti-CNKSR1 Antibody

Target: CNKSR1 (Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1))

Binding Specificity: AA 10-217

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNKSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hCNK1 aa. 10-217

Clone: 46-hCNK1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CNKSR1 antibody (ABIN968649)

Therrien, Wong, Kwan, Rubin: "Functional analysis of CNK in RAS signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 23, pp. 13259-63, (1999) (PubMed).

Therrien, Wong, Rubin: "CNK, a RAF-binding multidomain protein required for RAS signaling." in: Cell, Vol. 95, Issue 3, pp. 343-53, (1998) (PubMed).

Target Details for CNKSR1

Target: CNKSR1 (Connector Enhancer of Kinase Suppressor of Ras 1 (CNKSR1))

Alternative Name: hCNK1 (CNKSR1 Products)

Synonyms: zgc:77200 antibody, CNKSR1 antibody, cnk antibody, ksr antibody, cnk1 antibody, CNK antibody, CNK1 antibody, KSR antibody, BC037758 antibody, connector enhancer of kinase suppressor of Ras 1 antibody, connector enhancer of kinase suppressor of Ras 1 L homeolog antibody, cnksr1 antibody, CNKSR1 antibody, cnksr1.L antibody, Cnksr1 antibody

Background: Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. These proteins relay signals from Tyr-kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in Drosophila in a genetic screen that identified mutations that suppress constitutively active Ras mutants. Connector enhancer of KSR (CNK) was found in a similar screen to identify mutations that enhance the KSR- dependent phenotype. Drosophila CNK contains a sterile alpha motif (SAM), a conserved region in CNK (CRIC), and a PDZ domain in the N-terminal region, a proline-rich and a plecstrin homology (PH) domain in the central region, and a C-terminal Pro-rich domain. The human homologue of CNK (hCNK1) contains similar N-terminal and central domains, but is 713 amino acids in length compared to 1557 amino acids for Drosophila CNK. In Drosophila, the N-terminal region of CNK facilitates binding to RAS, while the C-terminal region inhibits RAS- and RAF-dependent signaling. Thus, CNK may be important for regulation of both RAS- and RAF-dependent signaling.
Synonyms: Connector enhancer of KSR

Molecular Weight: 79-100 kDa