Caspase 8 antibody

Catalog No. ABIN969003

This anti-Caspase 8 antibody is a Mouse Monoclonal antibody detecting Caspase 8 in WB, IHC, ELISA and FACS. Suitable for Human, Mouse, Rat and Monkey. This Primary Antibody has been cited in 2+ publications.

Quick Overview for Caspase 8 antibody (ABIN969003)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 1H11

Product Details anti-Caspase 8 Antibody

Purpose: CASP8 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CASP8 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Caspase 8 antibody (ABIN969003)

Fulda: "Caspase-8 in cancer biology and therapy." in: Cancer letters, Vol. 281, Issue 2, pp. 128-33, (2009) (PubMed).

Jani, Gobejishvili, Hote, Barve, Joshi-Barve, Kharebava, Suttles, Chen, McClain, Barve: "Inhibition of methionine adenosyltransferase II induces FasL expression, Fas-DISC formation and caspase-8-dependent apoptotic death in T leukemic cells." in: Cell research, Vol. 19, Issue 3, pp. 358-69, (2009) (PubMed).

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Molecular Weight: 26 kDa

Gene ID: 841

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades