Integrin beta 2 antibody

Catalog No. ABIN969014

Product Details anti-Integrin beta 2 Antibody

Target: Integrin beta 2 (ITGB2)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Purpose: CD18 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of CD18 expressed in E. Coli.

Clone: 10E12

Isotype: IgG2b

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Integrin beta 2 antibody (ABIN969014)

Chakrabarti, Patel: "The atypical zeta (zeta) isoform of protein kinase C regulates CD11b/CD18 activation in human neutrophils." in: Microcirculation (New York, N.Y. : 1994), Vol. 15, Issue 6, pp. 555-67, (2008) (PubMed).

Doisne, Castaigne, Deruyffelaere, Dieu-Nosjean, Chamot, Alcaide-Loridan, Charron, Al-Daccak: "The context of HLA-DR/CD18 complex in the plasma membrane governs HLA-DR-derived signals in activated monocytes." in: Molecular immunology, Vol. 45, Issue 3, pp. 709-18, (2007) (PubMed).

Li, Tang, Puan, Law, Tan: "The cytosolic protein talin induces an intermediate affinity integrin alphaLbeta2." in: The Journal of biological chemistry, Vol. 282, Issue 33, pp. 24310-9, (2007) (PubMed).

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 (ITGB2 Products)

Background:

Description: CD18, also known as ITGB2 (integrin beta chain beta 2). Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. CD18 is expressed by most leucocytes. Defects in this gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the same protein have been identified for this gene.

Aliases: LAD, MF17, LCAMB, LFA-1, ITGB2

Molecular Weight: 85kDa

Gene ID: 3689

HGNC: 3689

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation