Estrogen Receptor alpha antibody

Catalog No. ABIN969119

The Mouse Monoclonal anti-Estrogen Receptor alpha antibody has been validated for WB, IHC, ELISA and ICC. It is suitable to detect Estrogen Receptor alpha in samples from Human. There are 2+ publications available.

Quick Overview for Estrogen Receptor alpha antibody (ABIN969119)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 6B6

Product Details anti-Estrogen Receptor alpha Antibody

Purpose: ESR1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ESR1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Estrogen Receptor alpha antibody (ABIN969119)

Deegan, Seldeen, McDonald, Bhat, Farooq: "Binding of the ERalpha nuclear receptor to DNA is coupled to proton uptake." in: Biochemistry, Vol. 49, Issue 29, pp. 5978-88, (2010) (PubMed).

Harsløf, Husted, Carstens, Stenkjaer, Langdahl: "Genotypes and haplotypes of the estrogen receptor genes, but not the retinoblastoma-interacting zinc finger protein 1 gene, are associated with osteoporosis." in: Calcified tissue international, Vol. 87, Issue 1, pp. 25-35, (2010) (PubMed).

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

Molecular Weight: 66 kDa

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly