GRK2 antibody

Catalog No. ABIN969178

This Mouse Monoclonal antibody specifically detects GRK2 in WB, ELISA, IHC and ICC. It exhibits reactivity toward Human, Mouse, Rat and Monkey and has been mentioned in 2+ publications.

Quick Overview for GRK2 antibody (ABIN969178)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 3F8

Product Details anti-GRK2 Antibody

Purpose: GRK2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GRK2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GRK2 antibody (ABIN969178)

Sterne-Marr, Leahey, Bresee, Dickson, Ho, Ragusa, Donnelly, Amie, Krywy, Brookins-Danz, Orakwue, Carr, Yoshino-Koh, Li, Tesmer: "GRK2 activation by receptors: role of the kinase large lobe and carboxyl-terminal tail." in: Biochemistry, Vol. 48, Issue 20, pp. 4285-93, (2009) (PubMed).

Chen, Long, Wu, Jiang, Ma: "EGF transregulates opioid receptors through EGFR-mediated GRK2 phosphorylation and activation." in: Molecular biology of the cell, Vol. 19, Issue 7, pp. 2973-83, (2008) (PubMed).

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. (provided by RefSeq)Tissue specificity: Expressed in peripheral blood leukocytes

Molecular Weight: 80 kDa

Gene ID: 156

UniProt: P25098

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling