IL-1 beta antibody

Catalog No. ABIN969215

There are 2+ publications for this product available. The Mouse Monoclonal anti-IL-1 beta antibody is suitable to detect IL-1 beta in samples from Human. It has been validated for IHC, ELISA and ICC.

Quick Overview for IL-1 beta antibody (ABIN969215)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-1 beta antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 3A6

Product Details anti-IL-1 beta Antibody

Purpose: IL1B Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human IL1B expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-IL-1 beta antibody (ABIN969215)

Mohideen, Capili, Bilimoria, Yamada, Bonni, Lima: "A molecular basis for phosphorylation-dependent SUMO conjugation by the E2 UBC9." in: Nature structural & molecular biology, Vol. 16, Issue 9, pp. 945-52, (2009) (PubMed).

Kuo, Bentsi-Barnes, Barlow, Bae, Pisarska: "Sumoylation of forkhead L2 by Ubc9 is required for its activity as a transcriptional repressor of the Steroidogenic Acute Regulatory gene." in: Cellular signalling, Vol. 21, Issue 12, pp. 1935-44, (2009) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL1B

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 31 kDa

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome