LCK antibody (AA 39-52)

Catalog No. ABIN987272

The Goat Polyclonal anti-LCK antibody is suitable to detect LCK in samples from Human, Rat and Pig. It has been validated for WB and ELISA.

Quick Overview for LCK antibody (AA 39-52) (ABIN987272)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-LCK Antibody

Binding Specificity: AA 39-52

Purpose: LCK (aa39-52)

Sequence: RNGSEVRDPL VTYE

Specificity: This antibody is not expected to cross-react with other tyrosine protein kinases. Reported variants represent identical protein: NP_005347.3, NP_001036236.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RNGSEVRDPLVTYE, from the internal region (near N Terminus) of the protein sequence according to NP_005347.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human and Rat Thymus lysates (calculated MW of 58.0 kDa according to NP_005347.3). Recommended concentration: 0.1-0.3 μg/mL. Approx 60 kDa band observed in Pig Bone Marrow and Spleen lysates (calculated MW of 58.2

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: LCK

Background: LCK, lymphocyte-specific protein tyrosine kinase, LSK, YT16, p56lck, pp58lck, OTTHUMP00000008640, OTTHUMP00000008740, OTTHUMP00000008741, T-lymphocyte specific protein tyrosine kinase p56lck, leukocyte C-terminal Src kinase, lymphocyte cell-specific prote

Gene ID: 3932, 16818, 313050

NCBI Accession: NP_005347

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling