GPD1 antibody (Biotin)

Catalog No. ABIN99898

Product Details anti-GPD1 Antibody (Biotin)

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This GPD1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Glycerol-3-Phosphate-Dehydrogenase from other sources is unknown.

Purity: Anti-Glycerol-3-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle].

Endotoxin Level: Low Endotoxin : No

Immunogen: Glycerol-3-Phosphate-Dehydrogenase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Glycerol-3-Phosphate Dehydrogenase has been assayed against 1.0 µg of Glycerol-3-Phosphate-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1.000 to 1:5.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for GPD1

Target: GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))

Alternative Name: Glycerol-3-Phosphate Dehydrogenase (GPD1 Products)

Synonyms: GPDH antibody, Gpd3 antibody, GPD-C antibody, GPDH-C antibody, HTGTI antibody, CG9042 antibody, DROGPDHA antibody, DmG3PDH antibody, Dmel\\CG9042 antibody, G3PDH antibody, G3pdh antibody, GAPDH antibody, GPD antibody, GPDA antibody, GPDH-1 antibody, Gdh antibody, Gpd antibody, alpha-GPD antibody, alpha-GPDH antibody, alpha-GPDH-1 antibody, alpha-Gpdh antibody, alphaGPDH antibody, alphaGpd antibody, alphaGpdh antibody, alphaGpdh-1 antibody, gpdh antibody, gpdh-1 antibody, sn-Gpdh antibody, AI747587 antibody, Gdc-1 antibody, Gdc1 antibody, mKIAA4010 antibody, gpd1 antibody, wu:fc30a07 antibody, zgc:63859 antibody, MGC79676 antibody, GPD1 antibody, LG3P antibody, zgc:112197 antibody, Gpd1 antibody, gpd1h antibody, gpd1l antibody, wu:fc58b05 antibody, zgc:66051 antibody, zgc:85742 antibody, glycerol-3-phosphate dehydrogenase 1 antibody, Glycerol-3-phosphate dehydrogenase [NAD(+)] antibody, glycerol-3-phosphate dehydrogenase antibody, Glycerol 3 phosphate dehydrogenase antibody, glycerol-3-phosphate dehydrogenase 1 (soluble) antibody, glycerol-3-phosphate dehydrogenase 1 L homeolog antibody, glycerol-3-phosphate dehydrogenase 1c antibody, glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic antibody, glycerol-3-phosphate dehydrogenase 1a antibody, glycerol-3-phosphate dehydrogenase 1b antibody, Gpd1 antibody, gpdh-1 antibody, GPD1 antibody, Tb11.02.5280 antibody, Gpdh antibody, gpd1.L antibody, gpd1c antibody, gpd1 antibody, LOC101071719 antibody, gpd1a antibody, gpd1b antibody

Background: Anti-Glycerol-3-Phosphate Dehydrogenase recognizes the enzyme glycerol-3-phosphate dehydrogenase, a major component of lipid biosynthesis. Glyercol-3-phosphate dehydrogenase catalyzes the reduction of dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate. In addition, it assists in maintaining the redox potential across the inner mitochondrial membrane in glycloysis. Glycerol-3-phosphate dehydrogenase can be found in the cytosol and the inner mitochondrial membrane. Anti-Glycerol-3-Phosphate Dehydrogenase is ideal for investigators interested in metabolism, cancer, and cardiovascular diseases. Synonyms: EC 1.1.1.8 antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody

Gene ID: 100339469, 3043365

UniProt: P08507