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Western Blot Antibodies

Western blot (WB) antibodies are optimized for the detection of denatured proteins and validated to deliver clear, specific bands at the expected molecular weight. Use the filters below to quickly identify primary antibodies matched to your target, species, and experimental requirements.

Western Blot Antibody Validation

Antibodies for Western blot must reliably detect target proteins under denaturing conditions. Products in this category are validated for WB applications and selected for specific band detection at the expected molecular weight. Where available, validation data include experimental images, independent testing, and literature references to support antibody performance.

Western Blot Considerations

Protein denaturation during Western blotting can alter epitope structure, making antibody selection critical. Antibodies validated for WB are optimized to recognize linearized epitopes and reduce non-specific binding.

Monoclonal antibodies provide high specificity and consistent results, while polyclonal antibodies can offer stronger signal due to multi-epitope recognition. Recombinant antibodies combine defined specificity with improved reproducibility across experiments.

Western Blotting: Principles, Protocols, and Troubleshooting

Learn more about Western blotting, including protocols and troubleshooting

Use the filters below to narrow down Western blot antibodies by target, species, and experimental requirements.

529,062 results

DUSP5 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2F3 unconjugated
Pubmed 1 reference
Catalog No. ABIN560667
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated
Pubmed 6 references
Catalog No. ABIN559756
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
AMBP Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3F1 unconjugated
Pubmed 1 reference
Catalog No. ABIN559860
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
B2M Reactivity: Human WB, IF, ELISA, IHC (p), RNAi, PLA Host: Mouse Monoclonal 3F9-2C2 unconjugated
Pubmed 1 reference
Catalog No. ABIN560019
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
AXL Reactivity: Human WB, ELISA, RNAi Host: Mouse Monoclonal 6C8 unconjugated
Pubmed 1 reference
Catalog No. ABIN560017
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
RUNX2 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1D8 unconjugated
Pubmed 2 references
Catalog No. ABIN560185
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
RHOA Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1B12 unconjugated
Pubmed 3 references
Catalog No. ABIN559931
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ARF5 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1B4 unconjugated
Pubmed 8 references
Catalog No. ABIN559926
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
BGN Reactivity: Human WB, IP, ELISA, IHC (p) Host: Mouse Monoclonal 4E1-1G7 unconjugated
Pubmed 1 reference
Catalog No. ABIN560056
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
DARS Reactivity: Human WB, ELISA, IHC (p), RNAi Host: Mouse Monoclonal 2F11 unconjugated
Pubmed 1 reference
Catalog No. ABIN560561
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
GTF2H1 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 1F12-1B5 unconjugated
Pubmed 1 reference
Catalog No. ABIN561177
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
GTF2I Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 3E2 unconjugated
Pubmed 1 reference
Catalog No. ABIN561180
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
BIRC5 Reactivity: Human WB, ELISA, IF, IP, RNAi Host: Mouse Monoclonal 5B10 unconjugated
Pubmed 2 references
Catalog No. ABIN559893
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
AKR1C4 Reactivity: Human WB, ELISA, IHC (p), IF Host: Mouse Monoclonal 2C11 unconjugated
Pubmed 1 reference
Catalog No. ABIN560358
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CTH Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal S51 unconjugated
Pubmed 7 references
Catalog No. ABIN560523
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CTH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4E1-1B7 unconjugated
Pubmed 21 references
Catalog No. ABIN560521
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CTNS Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5G6 unconjugated
Pubmed 2 references
Catalog No. ABIN560527
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ALB Reactivity: Human WB, ELISA, IP, IHC (p) Host: Mouse Monoclonal 1G12-1B3 unconjugated
Pubmed 3 references
Catalog No. ABIN559842
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CDH1 Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 3F4 unconjugated
Pubmed 1 reference
Catalog No. ABIN560274
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CSTF3 Reactivity: Human WB, ELISA, IHC (p), IF, RNAi Host: Mouse Monoclonal 1D4 unconjugated
Pubmed 1 reference
Catalog No. ABIN560513
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
ANXA5 Reactivity: Human WB, ELISA, IP, IF, IHC (p) Host: Mouse Monoclonal 1F4-1A5 unconjugated
Pubmed 3 references
Catalog No. ABIN559879
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
BUB1B Reactivity: Human WB, ELISA, IHC (p), PLA, RNAi Host: Mouse Monoclonal 2G5 unconjugated
Pubmed 5 references
Catalog No. ABIN560109
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
CUX1 Reactivity: Human WB, ELISA, IF, IP, IHC (p) Host: Mouse Monoclonal 2A10 unconjugated
Pubmed 2 references
Catalog No. ABIN560539
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
XRCC6 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4C2-1A6 unconjugated
Pubmed 2 references
Catalog No. ABIN560973
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
GABBR1 Reactivity: Human WB, ELISA, IF, RNAi Host: Mouse Monoclonal 2D7 unconjugated
Pubmed 4 references
Catalog No. ABIN560976
$489.14
Plus shipping costs $50.00
100 μg
Shipping to: United States
Delivery in 11 to 14 Business Days
 
  • Type Primary
    • Primary
  • Target
    • Histone 3 (H3)
    • p53 (TP53)
    • EGFR
    • tau
    • AKT1
    • NF-kB p65 (NFkBP65)
    • Estrogen Receptor alpha (ESR1)
    • Bcl-2 (BCL2)
    • KIT
    • TNF alpha
    • RAF1
    • c-MYC (MYC)
    • FAK (PTK2)
    • Vimentin (VIM)
    • GFAP
    • RPS6KB1
    • HSP27 (HSPB1)
    • ErbB2/Her2
    • Cytokeratin 18 (KRT18)
    • HSP70
    • Albumin (ALB)
    • CDK1
    • C-JUN (JUN)
    • IL-6 (IL6)
    • Histone H4 (HISTH4)
    • ATF2
    • STAT3
    • EpCAM (EPCAM)
    • SMAD2
    • Caspase 9 (CASP9)
    • GAPDH
    • SNCA
    • Cytokeratin 19 (KRT19)
    • MEK1 (MAP2K1)
    • Interferon gamma (IFNG)
    • beta Catenin (CATNB)
    • E-cadherin (CDH1)
    • Caspase 3 (CASP3)
    • CREB1
    • HSPD1
    • CD31 (PECAM1)
    • Histone H2B
    • SOD1
    • BAD
    • GRP78 (HSPA5)
    • ERK1 (MAPK3)
    • Src
    • CD45 (PTPRC)
    • eIF4EBP1 (EIF4EBP1)
    • FGFR1
    Please use search to find more...
  • Application Western Blotting (WB)
    • Western Blotting (WB)
    • ELISA
    • Immunohistochemistry (IHC)
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunofluorescence (IF)
    • Flow Cytometry (FACS)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunocytochemistry (ICC)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunoprecipitation (IP)
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Enzyme Immunoassay (EIA)
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunofluorescence (fixed cells) (IF/ICC)
    • Dot Blot (DB)
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Immunochromatography (IC)
    • Functional Studies (Func)
    • Radioimmunoassay (RIA)
    • Neutralization (Neut)
    • Chromatin Immunoprecipitation (ChIP)
    • Coating (Coat)
    • Staining Methods (StM)
    • Immunostaining (ISt)
    • Fluorescence Microscopy (FM)
    • Immunoassay (IA)
    • Immunodiffusion (ID)
    • Proximity Ligation Assay (PLA)
    • Lateral Flow (LF)
    • Surface Plasmon Resonance (SPR)
    • Confocal Microscopy (CM)
    • Immunoelectrophoresis (IEP)
    • Live Cell Imaging (LCI)
    • Bio-Layer Interferometry (BLI)
    • Cytotoxicity Test (CyTox)
    • Indirect Immunofluorescence Assay (IFA)
    • RNA Interference (RNAi)
    • Antibody Array (AA)
    • Blocking Antibody (Inhibition)
    • Blocking Reagent (BR)
    • Blocking Peptide (BP)
    • ELISA (Capture)
    • Chemiluminescence Immunoassay (CLIA)
    • ChIP DNA-Sequencing (ChIP-seq)
    • In vivo Studies (in vivo)
    • Protein Assay (PrA)
    • Affinity Purification (AP)
    • Cellular Assay (CA)
    • Intracellular Flow Cytometry (ICFC)
    • Luminex Assay (LMNX)
    Please use search to find more...
  • Reactivity
    • Human
    • Mouse
    • Rat
    • Cow
    • Dog
    • Rabbit
    • Horse
    • Guinea Pig
    • Pig
    • Monkey
    • Zebrafish (Danio rerio)
    • Chicken
    • Bat
    • Sheep
    • Hamster
    • Xenopus laevis
    • Goat
    • Saccharomyces cerevisiae
    • Drosophila melanogaster
    • Arabidopsis thaliana
    • Virus
    • E. coli
    • Cat
    • Various Species
    • Plant
    • Human Immunodeficiency Virus (HIV)
    • Mammalian
    • Frog
    • Fish
    • Bacteria
    • Hepatitis C Virus (HCV)
    • Aequorea victoria
    • Oryza sativa
    • Chimpanzee
    • Non-Human Primate
    • Zea mays
    • Primate
    • C. elegans
    • Rhesus Monkey
    • SARS Coronavirus-2 (SARS-CoV-2)
    • Tag
    • Chemical
    • Chlamydomonas reinhardtii
    • Cynomolgus
    • Avian
    • Barley
    • Nicotiana tabacum
    • Arabidopsis
    • Influenza A Virus
    • Hepatitis B Virus (HBV)
    Please use search to find more...
  • Cited in publications
  • Images available
  • Independent Validation
  • Preservative free only
  • Carrier free only
  • Detection Method
    • Fluorometric
  • Host
    • Rabbit
    • Mouse
    • Goat
    • Sheep
    • Rat
    • Chicken
    • Guinea Pig
    • Human
    • Hamster
    • Alpaca
    • Mammalian Cells
    • Humanized
    • Chicken egg
    • Llama
    • Cell culture
    • Human Cells
    • Armenian Hamster
    • Cow
    • HEK-293F Cells
    • Chicken eggs
    • Golden Syrian Hamster
    • Horse
    • CHO Cells
    • Dog
    • Donkey
    • Carp
    • Fruit Fly (Drosophila melanogaster)
    • Phage Display
    • Pig
    • Tobacco (Nicotiana benthamiana)
  • Clonality
    • Polyclonal
    • Monoclonal
    • Chimeric
    • Bispecific
  • Clone
    • C1
    • C2
    • C3
    • C4
    • C5
    • C6
    • C10
    • C7
    • C11
    • D1
    • D4
    • C8
    • 1F6
    • 1D6
    • C12
    • D2
    • 2C4
    • 2B11
    • D3
    • 1F4
    • C9
    • 1C4
    • 1D2
    • 3G1
    • 1F10
    • 2D3
    • 1F3
    • 2E9
    • 2F11
    • 1A8
    • 1F11
    • 1A7
    • 1D4
    • 1E8
    • 1F8
    • 1G2
    • 1G5
    • 3F8
    • 3G4
    • 1A5
    • 1B2
    • 1D8
    • 1C1
    • 1E1
    • 2C1
    • 1A4
    • 2D7
    • 1A1
    • 1B4
    • 1B7
    Please use search to find more...
  • Binding Specificity
    • C-Term
    • N-Term
    • Center
    • Internal Region
    • Middle Region
    • AA 101-200
    • AA 1-100
    • AA 1-30
    • AA 51-150
    • AA 201-300
    • AA 151-250
    • AA 21-120
    • AA 301-400
    • AA 251-350
    • AA 1-50
    • AA 31-130
    • AA 401-500
    • AA 351-450
    • AA 121-220
    • AA 501-600
    • AA 131-230
    • AA 451-550
    • Extracellular
    • AA 1-80
    • AA 81-180
    • AA 61-160
    • AA 231-330
    • Extracellular Domain
    • AA 221-320
    • AA 41-140
    • AA 35-84
    • AA 71-120
    • AA 601-700
    • AA 161-260
    • Intracellular
    • AA 331-430
    • AA 51-100
    • AA 50-100
    • AA 181-280
    • AA 701-800
    • Cytosolic
    • AA 551-650
    • AA 1-300
    • AA 141-240
    • AA 1-110
    • AA 30-110
    • AA 1-200
    • AA 107-156
    • AA 251-300
    • AA 65-160
    Please use search to find more...
  • Conjugate
    • Un-conjugated
    • Biotin
    • FITC
    • HRP
    • PE
    • APC
    • Cy3
    • AbBy Fluor® 594
    • Cy5
    • AbBy Fluor® 350
    • AbBy Fluor® 488
    • AbBy Fluor® 555
    • AbBy Fluor® 647
    • Cy5.5
    • Cy7
    • AbBy Fluor® 750
    • AbBy Fluor® 680
    • Alexa Fluor 350
    • Alexa Fluor 405
    • Alexa Fluor 647
    • Alexa Fluor 750
    • Alexa Fluor 488
    • Alexa Fluor 594
    • Alexa Fluor 555
    • Alexa Fluor 680
    • CF®740
    • CF®488A
    • CF®405S
    • CF®568
    • CF®640R
    • CF®647
    • CF®594
    • PE-Cy5
    • PE-Cy7
    • PE-Cy5.5
    • PerCP
    • Atto 488
    • Atto 390
    • Atto 594
    • Alkaline Phosphatase (AP)
    • Peroxidase (POD)
    • DyLight 488
    • Texas Red (TR)
    • DyLight 680
    • DyLight 800
    • DyLight 405
    • DyLight 549
    • DyLight 649
    • CF®555
    • Rhodamine
    • His tag
  • Isotype
    • IgG
    • Ig Fraction
    • IgG1
    • IgG2a
    • IgG1 kappa
    • IgG2b
    • kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG2
    • IgM
    • IgY
    • IgG3
    • IgG kappa
    • IgM kappa
    • IgG3 kappa
    • IgG2c
    • IgG1 lambda
    • IgG Mix lambda
    • IgG2a lambda
    • IgG2c kappa
    • L234A
    • L235A
    • P329G
    • IgG2b lambda
    • IgG lambda
    • IgG4
    • IgG2 lambda
    • Ig kappa
    • IgG1/IgG2b
    • IgG2 kappa
    • lambda
    • IgA
    • IgA kappa
    • IgG Mix kappa
    • D265A
    • IgG1b kappa
    • IgG3 lambda
    • IgG IgA IgM Mix lambda
    • IgG1a
    • IgG4 kappa
    • IgM lambda
    • Ig
    • Ig Mix
    • IgG2c lambda
    • Fc Silenced
    • IgA2 kappa
    • IgE
    • IgE kappa
    • IgG IgA IgM Mix
    Please use search to find more...
  • Protein Type
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
    • Frozen
    • Lyophilized or Liquid
  • Fragment
    • single-domain Antibody (sdAb)
    • Fc fragment
    • Fab fragment
    • scFv fragment
    • F(ab')2 fragment
  • Grade
    • Verified
    • KD Validated
    • KO Validated
    • Carrier-free
    • Cell Culture Grade
    • In vivo Grade
    • KO Validated, KD Validated
    • Research Grade
    • ChIP-seq Grade
    • Animal-Free
    • GMP Grade
    • ChIP Grade
    • Calibrator Grade
    • MALS verified
    • Recent
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    • NSJ Bioreagents
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    • Rockland
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    • CUSABIO
    • Agrisera
    • Alomone Labs
    • Enogene Biotech
    • Antibodies Incorporated
    • Abiocode
    • OriGene
    • Wuhan Fine biotech
    • Elabscience
    • Synaptic Systems
    • Abeomics
    • RevMAb Biosciences
    • Active Motif
    • QED Bioscience
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    Please use search to find more...
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