anti-Cow (Bovine) BRCA1 antibody for Western Blotting

Recommended BRCA1 Antibody (supplied by: Log in to see )

Antigen
Breast Cancer 1 (BRCA1) Antibodies
  • BRCAI
  • BRCC1
  • BROVCA1
  • IRIS
  • PNCA4
  • PPP1R53
  • PSCP
  • RNF53
  • iris
  • pscp
  • brcai
  • brcc1
  • rnf53
  • brca1
  • BRCA1
  • LOC100224649
  • BRCA1, DNA repair associated
  • breast cancer 1
  • breast cancer 1, early onset
  • breast cancer 1 L homeolog
  • BRCA1
  • brca1
  • LOC755086
  • Brca1
  • brca1.L
Epitope
Middle Region
53
39
35
29
24
22
18
17
16
16
15
15
11
10
9
7
7
6
5
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
518
62
48
7
2
1
1
Host
Rabbit
402
126
4
3
Clonality
Polyclonal
Conjugate
This BRCA1 antibody is un-conjugated
21
16
14
5
5
5
5
5
5
5
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
309
166
147
120
77
74
68
58
22
21
21
12
7
6
4
3
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN2777874
$ 289.00
Plus shipping costs $45.00

General

Antigen Breast Cancer 1 (BRCA1) Antibodies
Epitope Middle Region
(53), (39), (35), (29), (24), (22), (18), (17), (16), (16), (15), (15), (11), (10), (9), (7), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(518), (62), (48), (7), (2), (1), (1)
Host Rabbit
(402), (126), (4), (3)
Clonality
Polyclonal
Conjugate This BRCA1 antibody is un-conjugated
(21), (16), (14), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(309), (166), (147), (120), (77), (74), (68), (58), (22), (21), (21), (12), (7), (6), (4), (3), (2), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-BRCA1 Antibody

Target Details BRCA1 Application Details Handling References for anti-BRCA1 antibody (ABIN2777874) Images
Blocking Peptide Blocking peptide for this product available: ABIN973871
Sequence DDLLDDGEIK EDTSFAENDI KESSAVFSKS VQKGELSRSP SPFTHTHLAQ
Predicted Reactivity Cow: 75%, Human: 100%, Mouse: 79%, Rat: 86%
Characteristics This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human BRCA1
Plasmids, Primers & others

Target Details BRCA1

Product Details anti-BRCA1 Antibody Application Details Handling References for anti-BRCA1 antibody (ABIN2777874) Images back to top
Antigen
Alternative Name BRCA1 (BRCA1 Antibody Abstract)
Background The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
Alias Symbols: BRCAI, BRCC1, IRIS, PSCP, RNF53, PNCA4, BROVCA1, PPP1R53
Protein Interaction Partner: RAD50, MED17, RAD51, YAP3, HDA3, SSN3, BEM4, GYP5, YAF9, HDAC2, MSH6, BAP1, SUPT5H, POLR2A, NPR1, NUP53, MET18, CDC21, MLP1, CTK1, MSH2, BACH1, DAN1, TMA22, BBC1, RPB4, SPT4, YGR053C, ATE1, YBP2, SLI15, RAD16, XRS2, RAD55, BUR2, BRIP1, ESR1, BECN1, HIST1H
Protein Size: 1863
Molecular Weight 208 kDa
Gene ID 672
NCBI Accession NM_007294, NP_009225
UniProt P38398
Pathways Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-BRCA1 Antibody Target Details BRCA1 Handling References for anti-BRCA1 antibody (ABIN2777874) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1863 AA

Restrictions For Research Use only

Handling

Product Details anti-BRCA1 Antibody Target Details BRCA1 Application Details References for anti-BRCA1 antibody (ABIN2777874) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-BRCA1 antibody (ABIN2777874)

Product Details anti-BRCA1 Antibody Target Details BRCA1 Application Details Handling Images back to top
Product cited in:

Evans, Shenton, Woodward, Lalloo, Howell, Maher: "Penetrance estimates for BRCA1 and BRCA2 based on genetic testing in a Clinical Cancer Genetics service setting: risks of breast/ovarian cancer quoted should reflect the cancer burden in the family." in: BMC cancer, Vol. 8, pp. 155, 2008

Images

Product Details anti-BRCA1 Antibody Target Details BRCA1 Application Details Handling References for anti-BRCA1 antibody (ABIN2777874) back to top
Supplier Images
Western Blotting (WB) image for anti-Breast Cancer 1 (BRCA1) (Middle Region) antibody (ABIN2777874) WB Suggested Anti-BRCA1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positiv...
Immunohistochemistry (IHC) image for anti-Breast Cancer 1 (BRCA1) (Middle Region) antibody (ABIN2777874) Western Blot with Cell line 293T (Embyonic human kidney cells) tissue