anti-Pig (Porcine) Chemokine (C-C Motif) Ligand 3-Like 1 antibody for Western Blotting

Recommended Chemokine (C-C Motif) Ligand 3-Like 1 Antibody (supplied by: Log in to see )

Antigen
Chemokine (C-C Motif) Ligand 3-Like 1 (CCL3L1) Antibodies
  • 464.2
  • D17S1718
  • G0S19-2
  • LD78
  • LD78BETA
  • MIP1AP
  • SCYA3L
  • SCYA3L1
  • C-C motif chemokine ligand 3 like 1
  • chemokine (C-C motif) ligand 3-like 1
  • CCL3L1
Reactivity
Pig (Porcine), Cow (Bovine)
48
8
7
3
1
Host
Rabbit
38
25
Clonality
Polyclonal
Conjugate
Un-conjugated
12
3
2
2
2
2
Application
ELISA, Western Blotting (WB)
50
44
7
5
2
1
1
1
1
1
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Catalog No. ABIN964604
$ 389.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0849469 ABIN964605 ELISA WB Rabbit IgG Log in to see Polyclonal
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General

Antigen Chemokine (C-C Motif) Ligand 3-Like 1 (CCL3L1) Antibodies
Reactivity Pig (Porcine), Cow (Bovine)
(48), (8), (7), (3), (1)
Host Rabbit
(38), (25)
Clonality
Polyclonal
Conjugate Un-conjugated
(12), (3), (2), (2), (2), (2)
Application ELISA, Western Blotting (WB)
(50), (44), (7), (5), (2), (1), (1), (1), (1), (1)
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Product details

Target details Application Details Handling Images
Specificity This product was Protein-A purified from monospecific antiserum by chromatography. This antibody is specific for swine CCL3L1 protein. A BLAST analysis was used to suggest cross-reactivity with CCL3L1 from bovine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against CCL3L1 from cat, dog, human, chimpanzee, baboon, bovine, and rat based on greater than 90% sequence homology. Cross-reactivity with CCL3L1 from other sources has not been determined.
Cross-Reactivity Cow (Bovine)
Characteristics Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha, other synonyms: ld78beta, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. In humans, CCL3L1 is encoded by a variable copy-number gene. The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages. Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5. CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.
Purification purified
Sterility Sterile filtered
Immunogen This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 70 amino acids of the mature swine CCL3L1 protein.
Immunogen Type: RecombinantProtein
Isotype IgG

Target details

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Antigen
Alternative Name CCL3L1 (CCL3L1 Antibody Abstract)
Background Chemokine (C-C motif) ligand 3-like 1 (CCL3L1), also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha; other synonyms: ld78beta, scya3l1, small inducible cytokine a3-like 1), is a member of the CC or beta chemokine subfamily that was originally purified from the conditioned media of an LPS-stimulated murine macrophage cell line. In humans, CCL3L1 is encoded by a variable copy-number gene. The CC family induces MCP1 and RANTES, and exhibits a variety of proinflammatory activities including chemotaxis, and functional and proliferative activation of leukocytes, lymphocytes, and macrophages. Its signal is transmitted through transmembrane receptors, CC chemokine receptors, CCR1, CCR3 and CCR5. CCL3L1/MIP-1 alpha acts as a chemoattractant to a variety of cells including monocytes, T cells, B cells and eosinophils. CCL3L1 binds to several chemokine receptors. In humans these receptors include chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, and binding of this protein to CCR5 inhibits HIV entry. In swine, CCL3L1 protein is 86% similar to CCL4 protein, and 73% similar to CCL5.
Synonyms: CCL3L1, Chemokine (C-C motif) ligand 3-like 1, macrophage inflammatory protein 1 alpha, MIP-1 alpha, ld78beta, scya3l1, small inducible cytokine a3-like 1
Gene ID 494459, 57527998
UniProt A1YM34
Pathways Cellular Response to Molecule of Bacterial Origin

Application Details

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Application Notes This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7.8 kDa in size corresponding to swine CCL3L1 protein by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: CCL3L1

Restrictions For Research Use only

Handling

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Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Chemokine (C-C Motif) Ligand 3-Like 1 (CCL3L1) antibody (ABIN964604) Western blot using protein-A purified anti-swine CCL3L1 antibody shows detection of r...