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|Antigen||Complement Factor H (CFH) Antibodies|
|Conjugate||This Complement Factor H antibody is un-conjugated Alternatives|
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
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Product Details anti-Complement Factor H AntibodyTarget Details Complement Factor H Application Details Handling Images
|Specificity||Gives a single arc when tested by IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) against human plasma and a known anti-human Factor H. The cross reactivity of this product against the sera of the species listed below has been assessed by double diffusion using 10ul of antisera against 10ul of species sera. These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative above may be observed when using different test conditions or techniques.|
|Characteristics||Sheep anti Factor H (Beta 1H), Sheep antibody to Human Factor H (Beta 1H)|
|Purification||Delipidated and if necessary, absorbed to monospecificity by use of solid-phase absorbants. An immunoglobulin fraction is then produced. The titer is adjusted so that inter-batch variation is within 10 %.|
|Sterility||0.2 μm filtered|
|Immunogen||Human 155 kDa Factor H purified from plasma, shown to be pure by IEP|
Target Details Complement Factor HProduct Details anti-Complement Factor H Antibody Application Details Handling Images back to top
|Alternative Name||Factor H (Beta 1H) (CFH Antibody Abstract)|
|Pathways||Complement System, Cellular Response to Molecule of Bacterial Origin|
Application DetailsProduct Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling Images back to top
|Application Notes||Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion, immunoelectrophoresis (IEP). The use of 3 % PEG 6000 with 1.3 % agarose in a suitable buffer (such as TBE or tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbent assays and Western blot and IHC has not been assessed but use in such assays should not necessarily be excluded. Each laboratory should determine an optimum working titer for use in its particular application. RID : 1 µL antiserum/cm2 gel vs. 1 µL neat - 1:10 dilution human plasma. Double Diffusion: 10 µL antiserum vs. 10 µL plasma. IEP: 101 µL antiserum vs. 1 µL plasma.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Images back to top
|Buffer||Glycine buffered saline, pH 7.4, 0.1 % Sodium azide, 0.1 % EACA, 0.01 % Benzamidine, 1 mM EDTA|
|Preservative||Sodium azide, EACA, Benzamidine|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Slight precipitation can occur on storage which may be removed by centrifugation, and should not affect performance characteristics.
Prepare working dilution only prior to immediate use.
|Storage Comment||Upon receipt, store at 2-8 °C.|