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|Antigen||Complement Factor I (CFI) Antibodies|
|Conjugate||This Complement Factor I antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|2 references available|
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Product Details anti-Complement Factor I AntibodyTarget Details Complement Factor I Application Details Handling References for anti-Complement Factor I antibody (ABIN181115) Images
|Specificity||This antibody recognises complement factor I, a member of the peptidase S1 family present in the blood plasma.|
|Characteristics||Synonyms: CFI, IF, KAF, AHUS3, C3BINA, C3b-INA, C3B/C4B inactivator|
|Purification||Affinity chromatography on Protein A|
|Immunogen||Native human factor I from serum.|
Target Details Complement Factor IProduct Details anti-Complement Factor I Antibody Application Details Handling References for anti-Complement Factor I antibody (ABIN181115) Images back to top
|Alternative Name||Complement Factor I (CFI Antibody Abstract)|
|Background||Factor I is an 88 kDa protein consisting of one polypeptide chain of 35.4 kDa and one of 27.6 kDa. Factor I is a serine protease which inactivates complement components C3b and C4b. C3b cleavage requires the presence of cofactors complement component (3b/4b) receptor 1 (CR1), membrane cofactor protein or factor H, while C4b cleavage requires complement component 4 binding protein or CR1. Factor I has no natural inhibitors. Dysfunctional factor I can lead to uncontrolled activation of the alternative complement pathway causing in systemic depletion of C3 and lower levels of factors B and H, resulting in recurrent pyogenic infections. Defects in CFI are also associated with haemolytic uraemic syndrome.Synonyms: AHUS3, C3B/C4B inactivator, C3BINA, C3b-INA, CFI, IF, KAF|
Application DetailsProduct Details anti-Complement Factor I Antibody Target Details Complement Factor I Handling References for anti-Complement Factor I antibody (ABIN181115) Images back to top
ELISA: 1/3000 - 1/4000. Western Blot. Functional Assays. Immunohistochemistry on frozen sections. Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Complement Factor I Antibody Target Details Complement Factor I Application Details References for anti-Complement Factor I antibody (ABIN181115) Images back to top
|Buffer||Borate buffered saline pH 8.4 containing 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-Complement Factor I antibody (ABIN181115)Product Details anti-Complement Factor I Antibody Target Details Complement Factor I Application Details Handling Images back to top
Nilsson, Karpman, Vaziri-Sani, Kristoffersson, Salomon, Provot, Fremeaux-Bacchi, Trouw, Blom: "A mutation in factor I that is associated with atypical hemolytic uremic syndrome does not affect the function of factor I in complement regulation." in: Molecular immunology, Vol. 44, Issue 8, pp. 1835-44, 2006
Dragon-Durey, Frémeaux-Bacchi: "Atypical haemolytic uraemic syndrome and mutations in complement regulator genes." in: Springer seminars in immunopathology, Vol. 27, Issue 3, pp. 359-74, 2005