anti-Human EGF antibody for Neutralization

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Reactivity
Human
382
186
109
20
17
9
4
2
2
1
1
1
Host
Rabbit
369
149
32
26
6
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Neutralization (Neut), Western Blotting (WB)
418
282
160
101
54
32
30
30
27
27
23
16
14
7
7
5
4
3
2
1
1
Supplier
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Catalog No. ABIN465547
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.579589 ABIN2139666 Neut WB Rabbit IgG Log in to see Polyclonal 0
8.5 ABIN223519 Neut WB Rabbit IgG Log in to see Polyclonal 1
7 ABIN465856 ELISA Neut WB Biotin Rabbit Log in to see Polyclonal 0
4 ABIN242161 Neut ELISA WB Rabbit Log in to see Polyclonal 0
4 ABIN5506793 Neut Rabbit IgG AA 971-1023 Log in to see R001 0
1 ABIN346557 Neut ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN2257864 Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1983159 FACS Neut WB Mouse IgG1 AA 971-1023 Log in to see MM0255-4T32 0
1 ABIN2260959 Neut ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260962 Neut ELISA WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260960 Neut ELISA WB APC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260961 Neut ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260964 Neut ELISA WB PE Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260963 Neut ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN2260958 Neut ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5688983 ELISA Neut WB Rabbit Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Reactivity Human
(382), (186), (109), (20), (17), (9), (4), (2), (2), (1), (1), (1)
Host Rabbit
(369), (149), (32), (26), (6), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Neutralization (Neut), Western Blotting (WB)
(418), (282), (160), (101), (54), (32), (30), (30), (27), (27), (23), (16), (14), (7), (7), (5), (4), (3), (2), (1), (1)
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling Images
Specificity Human EGF
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human EGF.

Type of Immunogen: Cell extract
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Gene ID 1950
UniProt P01133
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling Images back to top
Application Notes Approved: ELISA (0.5 - 2 μg/mL), Neut (0.25 - 0.35 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL-0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling back to top
Supplier Images
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 2)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 3)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 4)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 5)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 6)
 image for anti-Epidermal Growth Factor (EGF) antibody (ABIN465547) anti-Epidermal Growth Factor (EGF) antibody (Image 7)