Mouse IgG1, kappa isotype control (DyLight 488)
FACS, WB, ICC, IHC (fro), IHC (p)
Monoclonal
MOPC-21
IgG1 kappa
DyLight 488
1 image
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- Target See all IgG1, kappa products
- IgG1, kappa
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- DyLight 488
- Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) with unknown specificity has been confirmed as a good negative control with human and rat species, based on multiple testing on rat and human tissues.
- No Cross-Reactivity
- Human, Rat
- Purification
- Purified antibody is conjugated with DyLight® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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Clone
- MOPC-21
- Isotype
- IgG1 kappa
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Mouse IgG1, kappa isotype control (DyLight 650)
FACS, WB, ICC, IHC (fro), IHC (p) Monoclonal MOPC-21 IgG1 kappa DyLight 650
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgG1, kappa
- Alternative Name
- IgG1 kappa (IgG1, kappa Products)
- Target Type
- Antibody
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