Mouse IgG2b,kappa isotype control (DyLight 650)
FACS, WB, ICC, IHC (fro), IHC (p)
Monoclonal
MPC-11
IgG2b kappa
DyLight 650
1 image
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- Target
- IgG2b,kappa
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Host
- Mouse
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Clonality
- Monoclonal
- Conjugate
- DyLight 650
- Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This mouse IgG2b (kappa) monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.
- No Cross-Reactivity
- Human, Mouse, Rat
- Purification
- Purified antibody is conjugated with DyLight® 650 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- KLH-coupled trinitrophenol
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Clone
- MPC-11
- Isotype
- IgG2b kappa
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Mouse IgG2b,kappa isotype control (DyLight 488)
FACS, WB, ICC, IHC (fro), IHC (p) Monoclonal MPC-11 IgG2b kappa DyLight 488
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- Application Notes
- Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/mL of incubation mixture), change the conditions of your experiment to reduce the background.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgG2b,kappa
- Alternative Name
- IgG2b kappa
- Target Type
- Antibody
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