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|Antigen||Cardiotrophin 1 (CTF1) Antibodies|
|Conjugate||This Cardiotrophin 1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)
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Product Details anti-Cardiotrophin 1 AntibodyTarget Details Cardiotrophin 1 Application Details Handling Images
|Specificity||This antibody reacts with Human Cardiotrophin-1|
|Immunogen||Highly pure (>98%) recombinant hCardiotrophin-1.|
Target Details Cardiotrophin 1Product Details anti-Cardiotrophin 1 Antibody Application Details Handling Images back to top
|Alternative Name||Cardiotrophin-1 (CTF1 Antibody Abstract)|
|Background||Cardiotrophin-1 (CT-1), a cardiac hypertrophic factor, is a 21.5 kDa protein member of the IL-6 cytokine family. CT-1 is associated with the pathophysiology of heart diseases, including hypertension, myocardial infarction, valvular heart disease, and congestive heart failure. The protein exerts its cellular effects by interacting with the glycoprotein 130 (gp130)/leukemia inhibitory factor receptor beta (LIFR) heterodimer. In addition, CT-1 activates phosphatidylinositol 3-kinase (PI-3 kinase) in cardiac myocytes and enhances transcription factor NF kappa B DNA -binding activities. CT-1 is highly expressed in the heart, skeletal muscle, prostate and ovary and to lower levels in lung, kidney, pancreas, thymus, testis and small intestine.Synonyms: CT-1, CTF1|
Application DetailsProduct Details anti-Cardiotrophin 1 Antibody Target Details Cardiotrophin 1 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhCardiotrophin-1 (3 ng/mL), a concentration of 0.04-0.09 μg/mL of this antibody isrequired. ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can beused at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Cardiotrophin 1 Antibody Target Details Cardiotrophin 1 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|