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|Antigen||Ciliary Neurotrophic Factor (CNTF) Antibodies|
|Reactivity||Rat (Rattus) Alternatives|
|Conjugate||This CNTF antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-CNTF AntibodyTarget Details CNTF Application Details Handling Images
E.coli derived recombinant Rat CNTF.
Type of Immunogen: Cell extract
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|Alternative Name||CNTF (CNTF Antibody Abstract)|
Name/Gene ID: CNTF
Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF
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Approved: ELISA (0.5 - 2 μg/mL), IHC, Neut (2 - 5 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat CNTF (0.15 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat CNTF (LS-C104736) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
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|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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