anti-Human SRC antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended SRC Antibody (supplied by: Log in to see )

Antigen
V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
  • AW259666
  • pp60c-src
  • C-src1
  • CG7524
  • D-Src64B
  • D-src
  • DSRC64
  • DSrc
  • DSrc64
  • DSrc64B
  • Dm SRC1
  • DmHD-358
  • Dmel\CG7524
  • Dsrc
  • Dsrc64
  • Dsrc64B
  • HD-358
  • MRE5
  • SRC 64B
  • Src
  • Src1
  • Src64
  • c-src
  • dSrc
  • dsrc
  • src
  • src1
  • src64
  • src64B
  • src64b
  • ASV
  • SRC1
  • c-SRC
  • p60-Src
  • c-Src
  • PP60C-SCR
  • SDR
  • SRC proto-oncogene, non-receptor tyrosine kinase
  • uncharacterized LOC5574802
  • proto-oncogene tyrosine-protein kinase src
  • Rous sarcoma oncogene
  • Src oncogene at 64B
  • SRC
  • LOC5574802
  • CpipJ_CPIJ010659
  • Src
  • Src64B
Reactivity
Human, Mouse (Murine), Rat (Rattus)
429
239
193
30
18
16
15
14
9
7
6
6
5
4
4
2
1
1
1
1
1
1
1
Host
Rabbit
374
77
1
1
Clonality
Polyclonal
Conjugate
This SRC antibody is un-conjugated
19
19
15
14
13
13
3
3
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
383
217
153
56
44
32
26
21
16
9
7
5
3
3
1
Options
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Catalog No. ABIN738171
$ 306.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN712338 IF (p) FITC Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN712336 IF (p) Cy5.5 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN712337 IF (p) Cy7 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN712334 IF (p) Cy3 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN712335 IF (p) Cy5 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN879250 IF (p) Alexa Fluor 488 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN879249 IF (p) Alexa Fluor 350 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN879253 IF (p) Alexa Fluor 555 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN879252 IF (p) Alexa Fluor 647 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN2814994 IF (p) Alexa Fluor 594 Rabbit IgG pTyr215 Log in to see Polyclonal 0
1 ABIN916357 IF (p) Alexa Fluor 488 Rabbit IgG Log in to see Polyclonal 0
1 ABIN916361 IF (p) Alexa Fluor 555 Rabbit IgG Log in to see Polyclonal 0
1 ABIN916358 IF (p) Alexa Fluor 647 Rabbit IgG Log in to see Polyclonal 0
1 ABIN916360 IF (p) Alexa Fluor 350 Rabbit IgG Log in to see Polyclonal 0
1 ABIN738176 IF (p) Cy5.5 Rabbit IgG Log in to see Polyclonal 0
1 ABIN738174 IF (p) Cy3 Rabbit IgG Log in to see Polyclonal 0
1 ABIN738175 IF (p) Cy5 Rabbit IgG Log in to see Polyclonal 0
1 ABIN738178 IF (p) FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN738177 IF (p) Cy7 Rabbit IgG Log in to see Polyclonal 0
1 ABIN2814991 IF (p) Alexa Fluor 594 Rabbit IgG Log in to see Polyclonal 0

General

Antigen V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(429), (239), (193), (30), (18), (16), (15), (14), (9), (7), (6), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(374), (77), (1), (1)
Clonality
Polyclonal
Conjugate This SRC antibody is un-conjugated
(19), (19), (15), (14), (13), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(383), (217), (153), (56), (44), (32), (26), (21), (16), (9), (7), (5), (3), (3), (1)
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Product Details anti-SRC Antibody

Target Details SRC Application Details Handling Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human SRC
Isotype IgG

Target Details SRC

Product Details anti-SRC Antibody Application Details Handling Images back to top
Antigen
Alternative Name Src (SRC Antibody Abstract)
Background

c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr --> Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Synonyms: ASV, Avian sarcoma virus, c SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, cSrc, EC 2.7.10.2, Neuronal CSRC tyrosine specic protein kinase, Neuronal SRC, Oncogene SRC, OTTHUMP00000030931, OTTHUMP00000174476, OTTHUMP00000174477, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, protooncogene SRC Rous sarcoma, Src, SRC Oncogene, SRC_MOUSE, SRC1, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog.

Gene ID 6714
Pathways JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling

Application Details

Product Details anti-SRC Antibody Target Details SRC Handling Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-SRC Antibody Target Details SRC Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-SRC Antibody Target Details SRC Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) antibody (ABIN738171) Paraformaldehyde-fixed, paraffin embedded rat brain, Antigen retrieval by boiling in ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) antibody (ABIN738171) Paraformaldehyde-fixed, paraffin embedded mouse testis, Antigen retrieval by boiling ...