anti-Frog STAT1 antibody for Intracellular Staining

Recommended STAT1 Antibody (supplied by: Log in to see )

Antigen
Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
  • CANDF7
  • ISGF-3
  • STAT91
  • 2010005J02Rik
  • AA408197
  • XStat1
  • STAT4
  • DD6G4-4
  • STAT
  • STAT1
  • dZ182H3.3
  • si:by134g18.2
  • si:by51f19.1
  • si:dz182h3.3
  • si:dz199m19.1
  • si:dz73o4.2
  • signal transducer and activator of transcription 1
  • signal transducer and activator of transcription 1 L homeolog
  • signal transducer and activator of transcription 1, 91kDa
  • signal transducer and activator of transcription 4
  • STAT1
  • Stat1
  • stat1.L
  • stat4
Epitope
AA 1-194, N-Term
110
80
55
27
25
21
17
16
15
10
10
9
8
8
7
6
6
5
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
648
323
266
41
34
27
26
23
18
15
11
9
9
5
4
3
3
2
2
2
1
1
1
Host
Mouse
440
199
19
15
3
1
1
Clonality
Monoclonal
Conjugate
This STAT1 antibody is conjugated to Alexa Fluor 647
19
18
15
14
13
13
10
7
7
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
2
2
1
1
1
1
Application
Intracellular Staining (ICS)
562
233
186
129
107
55
52
29
26
11
9
7
5
5
2
1
1
1
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Catalog No. ABIN1176938
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176937 ICS PE Mouse IgG1 AA 1-194, N-Term Log in to see 1-Stat1 1

General

Antigen Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies
Epitope AA 1-194, N-Term
(110), (80), (55), (27), (25), (21), (17), (16), (15), (10), (10), (9), (8), (8), (7), (6), (6), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Dog (Canine), Frog, Human, Mouse (Murine), Rat (Rattus)
(648), (323), (266), (41), (34), (27), (26), (23), (18), (15), (11), (9), (9), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(440), (199), (19), (15), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This STAT1 antibody is conjugated to Alexa Fluor 647
(19), (18), (15), (14), (13), (13), (10), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(562), (233), (186), (129), (107), (55), (52), (29), (26), (11), (9), (7), (5), (5), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-STAT1 Antibody

Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN1176938) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Stat1 aa. 1-194
Clone 1-Stat1
Isotype IgG1
Plasmids, Primers & others

Target Details STAT1

Product Details anti-STAT1 Antibody Application Details Handling References for anti-STAT1 antibody (ABIN1176938) Images back to top
Antigen
Alternative Name Stat1 (STAT1 Antibody Abstract)
Background Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat1 and Stat2 are components of the ISGF3 (Interferon-Stimulated Gene Factor 3) complex, which is the primary transcription activator induced by the binding of the interferon to a specific cell-surface receptor. Stat1 has two alternatively spliced isoforms, 91-kDa Stat1alpha and 84 kDa Stat1beta, Stat1alpha has 38 additional C-terminal amino acids. In response to the binding of IFNalpha, IFNgamma, EGF, PDGF, or CSF-1 to their respective receptors, the Stat1 subunits become tyrosine-phosphorylated at Y701, and the complex is translocated to the nucleus. This results in the formation of an active complex that includes the DNA-binding p48 subunit. This complex is responsible for modulating the transcription of the interferon-stimulated genes (ISGs). Thus, phosphorylation of Y701 in Stat1 occurs in response to growth factors and cytokines, and is essential for normal transcriptional activity of the ISGF3 complex. The 1/Stat1 monoclonal antibody recognizes the N-terminus of human Stat1 (both isoforms), regardless of phosphorylation status.
Pathways JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events

Application Details

Product Details anti-STAT1 Antibody Target Details STAT1 Handling References for anti-STAT1 antibody (ABIN1176938) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details References for anti-STAT1 antibody (ABIN1176938) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-STAT1 antibody (ABIN1176938)

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling Images back to top
Product cited in:

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, 1996

Images

Product Details anti-STAT1 Antibody Target Details STAT1 Application Details Handling References for anti-STAT1 antibody (ABIN1176938) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-194), (N-Term) antibody (Alexa Fluor 647) (ABIN1176938) anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-194), (N-Term) antibody (Alexa Fluor 647)