|Antigen||Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) Antibodies|
|Epitope||AA 2-230 Alternatives|
|Reactivity||Human, Monkey Alternatives|
|Conjugate||This STAT1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-STAT1 AntibodyTarget Details STAT1 Application Details Handling Images
|Purpose||Mouse IgG monoclonal antibody for STAT1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human,Monkey.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
|Characteristics||Mouse IgG monoclonal antibody for STAT1 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human,Monkey.|
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human STAT1 recombinant protein (Position: S2-A230). Human STAT1 shares 91.2% amino acid (aa) sequence identity with mouse STAT1.|
|Plasmids, Primers & others|
Target Details STAT1Product Details anti-STAT1 Antibody Application Details Handling Images back to top
|Alternative Name||STAT1 (STAT1 Antibody Abstract)|
Synonyms: Signal transducer and activator of transcription 1-alpha/beta, Transcription factor ISGF-3 components p91/p84, STAT1
Background: Signal transducer and activator of transcription 1 (STAT1) is a transcription factor which in humans is encoded by the STAT1 gene. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
|Pathways||JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-STAT1 Antibody Target Details STAT1 Handling Images back to top
Application details: Western blot|0.1-0.5&mu,g/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1&mu,g/mL Immunocytochemistry/Immunofluorescence|2&mu,g/mL Flow Cytometry|1-3&mu,g/1x106 cells
Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
|Restrictions||For Research Use only|
HandlingProduct Details anti-STAT1 Antibody Target Details STAT1 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.