TOLLIP ELISA Kit

Catalog No. ABIN1161909

Product Details TOLLIP ELISA Kit

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of human Toll-interacting protein, concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Analytical Method: Quantitative

Specificity: This assay recognizes recombinant and natural human. No significant cross-reactivity or interference was observed.

Components: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Application Details

Comment:

Gene Name: TOLLIP

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: The microtiter plate provided in this kit has been pre-coated with an antibody specific to . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Storage Comment: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Target Details for TOLLIP

Target: TOLLIP (Toll Interacting Protein (TOLLIP))

Alternative Name: Toll-interacting protein (TOLLIP Products)

Synonyms: IL-1RAcPIP ELISA Kit, 4930403G24Rik ELISA Kit, 4931428G15Rik ELISA Kit, fk22a02 ELISA Kit, im:7145391 ELISA Kit, wu:fk22a02 ELISA Kit, zgc:76985 ELISA Kit, TOLLIP ELISA Kit, GB17961 ELISA Kit, tollip ELISA Kit, tollip1 ELISA Kit, tollipa ELISA Kit, TOLIP ELISA Kit, toll interacting protein ELISA Kit, toll-interacting protein ELISA Kit, toll-interacting protein-like ELISA Kit, toll interacting protein L homeolog ELISA Kit, toll interacting protein S homeolog ELISA Kit, Toll-interleukine I receptor interacting protein I ELISA Kit, TOLLIP ELISA Kit, Tollip ELISA Kit, tollip ELISA Kit, LOC552034 ELISA Kit, LOC100169858 ELISA Kit, tollip.L ELISA Kit, tollip.S ELISA Kit, LOC100136122 ELISA Kit

Background: Synonyms: Homo sapiens,Human,Toll-interacting protein,TOLLIP
Subunit: Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2.
Function: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.

UniProt: Q9H0E2